ABSTRACT
Background: Recently much of attention is focused on the diastolic function of the heart. Since the systolic performance of the heart in hypertensive patients has been examined so far, but the study of the diastolic function of heart has emerged as a newer mode of study. Aim: To study non-invasively the diastolic function of left ventricle in patients with systemic hypertension. Materials and methods: Twenty patients either admitted or as outpatients in outpatients Department, Gandhi Hospital with isolated systemic hypertension. (HTN) who satisfied inclusion and exclusion criteria were taken up for the study with five age matched individuals who along served as controls assessed by Doppler Echo-cardiography and their findings were compared with five normotensive age matched controls. Results: Bedside post valsalva maneuver, this test was positive in 7 5% of the test subjects. Of the 25% who had a negative test, 40% had evidence of diastolic dysfunction as assessed by pulsed wave Doppler. Left ventricular hypertrophy was present in 55% of the patient subgroup and all (100%) of these patients had significant diastolic dysfunction. The rest of the 45% of the patients did not have left ventricular hypertrophy. 75% of these non-hypertrophic hypertensives had diastolic dysfunction as seen by PWD. Increased left ventricular mass index has been observed in 85% of the hypertensives but has not correlated statistically with either severity of blood pressure or diastolic filling variables or isovolumic relaxation time. The left atrial size an indirect indicator of LV diastolic function was increased in 50% of the hypertensive subjects when compared with the controls but the statistical C. Venkateshwarlu, B. Praveen Kumar, Md. Yousuf Khan. Evaluation of left ventricular diastolic function in hypertensives. IAIM, 2016; 3(10): 212-220. Page 213 difference was insignificant. LV diastolic function as assessed by PWD mitral flow velocity profile and isometric volume relaxation time was abnormal in 85% of the hypertensive subgroup and was highly significant statistically (P value <0.005). The deceleration time (MS) was similar in both the controls and the hypertensives making it an insignificant parameter for assessing LV diastolic function. The peak filling rate was reduced in the hypertensive subgroup and was of moderate statistical significance. Conclusion: Doppler Echocardiography, an easily available non invasive technique today, can be utilized for identifying hypertensives with diastolic dysfunction and thus treat this group with specific therapy (Beta Blockers) so as to arrest or reverse the pathological changes produced in left ventricle due to hypertension.
ABSTRACT
A simple, precise, rapid, selective, and economic reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed for the simultaneous estimation of Lopinavir and Ritonavir in marketed formulations. Chromatographic separation was achieved isocratically for the combination was done with a C18 column [ ODS UG column. 250mm× 4.5 mm] utilizing mobile phase of composition Acetonitrile and Phosphate buffer (60:40v/v, pH 3) the flow rate was 1.5ml/min and the eluates was monitored at 220nm. Lopinavir and Ritonavir were eluted with retention times of 2.1min and 4.0min respectively. The method was found to be linear over a range of 20-100 μg/ml for Lopinavir and Ritonavir. The method was validated according to the guidelines of International Conference on Harmonisation (ICH) and was successfully employed in the estimation of commercial formulations.